Role of class 1 Integron in prevalence extended-spectrum beta-lactamases of class D (oxa-2 , oxa-10) along with determining antibiotic resistance pattern in Pseudomonas strains isolated from clinical specimens

Eradicating infections is difficult due to the presence of genes on the introns, especially the beta-lactamase enzymes and The importance of their resistance is high.The purpose of this study was to determine the beta-lactamase genes (OXA-2 , OXA-10) , Integron class 1 and antibiotic resistance.

In this cross sectional descriptive study, 120 Pseudomonas isolates were collected from Shiraz Burn Hospital during summer of 1395. After confirmation by biochemical tests, The presence of beta-lactamase genes was evaluated by using antibiotic discs and PCR method.

78.5% of the isolates had OXA-10 gene, 77.57% of the OXA-2 gene, 34.58% containing Int1, and 95.38% of the samples were MDR.Maximum resistant was seen in cefepime(79.43%) and the minimum was for to tobramycin(10.28%).

Class1 integron, 2-oxa and 10-oxa beta-lactamase genes, did not show up a meaningful relationship at the same time.the production of beta-lactamase enhances the increasing resistance and their frequency is increasing according to the results of other studies. This will lead more control over the prescription and use of antibiotics.