The Antibacterial Effect of body extracts Ectomyelois ceratoniae (Lepidoptera: Pyralidae)on the Escherichia coli
In recent years, biotechnologists and entomologists have emphasized the bioengineering of insect proteins and the applied of their products in the treatment of diseases and modern medicine. The aim of this study was to evaluate the anti-bacterial and hemolytic activity of E. ceratoniae larvae. To stimulate carob moth larvae immune system, standard strains isolate B. bassiana, B. thuringiensis and E. coli were injected. The hemolytic activity of the extracted induced and non-induced hemolymph after 24 hours of injection, to two methods of optical absorption (A) and radial diffusion (RD) on fresh blood of human, sheep and hen; and the antimicrobial effect to radial diffusion method on E. coli was assayed. The results showed that the induction extract had antimicrobial activity in comparison with non-induced extracts. The highest antimicrobial activity was observed in E. coli-induced extract (sub filter extract at a concentration of 2 mg / ml) with an average of 22.7 ± 0.34 mm diameter of non-growth holes. The highest hemolytic activity was in hen blood with total mean 6/39 ± 1/24 in A method and 5/64 ±1/25 in RD method. The results of this study showed that the extract of the carob moth larvae had a very low hemolytic effect on red blood cells, and the antimicrobial activity of the extract on E. coli was different according to the type of induction pathogen. According to the results of this study, this insect can be a good candidate for further study in complementary studies of antimicrobial activity.
Journal of Animan Researches, Volume:31 Issue:4, 2019
483 - 497
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