The efficiency of the partial genome of Turnip mosaic virus (TuMV) on resistance induction in canola

Turnip mosaic virus (TuMV) a species of the genus Potyvirus in the family Potyviridae, is one of the most prevalent viruses of canola fields in Iran. So far, commercial resistant lines or varieties against TuMV have not been known in Iran. In this research, the possibility of inducing resistant to TuMV in transgenic canola lines carrying a short fragment of the coat protein gene of the virus was evaluated. Therefore, a 130 nucleotide fragment of TuMV coat protein gene, as sense or antisense orientations, were cloned in the pFGC5941 vector independently and then were transformed to canola cotyledonary (Hayola R-Line 401 variety) explants using Agrobacterium tumefaciens strain LBA4404. Selection and confirmation of transgenic T0 and T1 lines were carried out via PCR and glufosinate ammonium herbicide (selective marker) resistance assay under greenhouse conditions. After virus inoculation to T1 lines, resistance assay was done by the scoring system. The obtained results confirmed that transgenic lines with sense construction did not show any resistant. However, several transgenic lines with antisense construction showed a different level of resistant to TuMV including delay in symptoms appearance and a decrease in symptoms severity (0-30% of T1 progeny) or symptoms recovery (10-66% of T1 progeny). This is the first report of increased resistant against TuMV via transformation of a short viral fragment sequence with the antisense orientation in canola.