Nano-Selenium Loading on Structure of Lactobacillus and Evaluating its Antimicrobial Potency to Make Anti-Diarrhea Drug-Supplement

Escherichia coli E.coli is the most important species that causes dysentery. Present study was conducted to investigate the synergistic antimicrobial effects of Nano-selenium and lactobacillus on E.coli to suggest anti-diarrhea bio-drug. The antimicrobial effects of experimental treatments against E.coli were evaluated by disk diffusion and well diffusion methods as a measure of the growth inhibition zone. Moreover, the minimum inhibitory concentration MIC and minimum bactericidal concentration MBC were determined by micro-dilution method. The results of the well and disk diffusion methods showed that in both tests, the highest growth inhibition zone of the "Nano selenium-loaded lactobacillus" treatment on the standard strain of E.coli was 23/6 and 23/36 mm respectively. The results of MIC and MBC determination showed that in all experimental periods, "Nano selenium-loaded lactobacillus" and "Nano-selenium lactobacillus" treatments with 590/7 and 615/38 μg/ml for MIC and 955/32 and 1195/67 μg/ml for MBC had the lowest amounts compared to other treatments P<0.05. The results of this experiment confirmed the anti-diarrhea ability of "Nano selenium-loaded lactobacillus" treatment. Therefore, provided that this test be repeated in future studies and ensured the accuracy of these results, manufacturing of bio-drug with this formulation may be advised for prevention or treatment of diarrheal disease.