The effect of antibacterial properties of zinc oxide nanoparticles on total count of bacterial and Gram-negative bacterial in the fresh water aquariums with goldfish (Carassius auratus Linnaeus, 1758)

In recent years the using of population controller component for bacteria in the water of the fish is highly regarded. in this context, the selection of appropriate disinfectant with antibacterial property is very important. In this study zinc oxide nanoparticles synthesized by sol-gel method and the minimum bactericidal concentration (MBC) of zinc oxide nanoparticles were evaluated to standard methods broth macrodilution serial dilutions, to determine the bacterial population in the aquarium environment includes two steps aquarium with goldfish and aquarium without fish. In the first step, concentrations of 0, 2 and 4 of the MBC and in the second step, concentrations of 0, 2, 4 and 8 of the MBC at special times added to aquarium water and antibacterial nanoparticles of zinc oxide were studied. MBC were measured 0.35 ϻg/ ml for bacterial populations of aquarium environment. The antibacterial activity of nanoparticle zinc oxide was observed, the total count of bacterial reduced in aquarium water with goldfish by increasing the concentrations of nanoparticles and increased contact time. The process of reducing the total count of bacterial in concentrations of 4 to MBC were observed, as well as the reduction of gram-negative bacterial in aquariums environment in both of 2 and 4 of MBC almost to the same extent was observed (no significant differences P>0.05).