Genetic Differentiation of Pterocarya fraxinifolia (Lam.) Spach populations In Iran using ISSR markers

Pterocarya fraxinifolia is an ancient tree in north of Iran that have been recently reported two small stand of this species in west of Iran, Lorestan and Ilam province. In this study, population diversity and genetic differentiation of west populations and their vulnerability were assessed using ISSR markers. Eight to ten trees were selected from two west population as well as four populations of north of Iran, DNA extracted from leaf and genetic diversity was evaluated. AMOVA result indicated that the value of intra and inter population diversity is 88% and 12%, respectively. Shannon index is 0.157 and mean of heterozygosity is variable 0.064 to 0.119. Based on neighbor joining algorithm, the under study populations were split to two main groups that the west populations (Ilam and Lorestan) as well as Savadkouh were comprised the main group 1. Also, PCoA analysis showed clearly differentiation of Lorestan population from other under study populations.