Effect of Lasalocid on rumen ecosystem and fermentation parameters in Ghezel sheep

This study was carried out to determine of the effects of lasalocid on ruminal fermentation parameters in Ghezel sheep. Sixteen male Ghezel sheep were used allocated in completely randomized design. The diet containing of 2.9 ME (Mcal/kg DM) and 150 g/kg DM CP was formulated based on NRC requirement consisting of 400 g kg-1DM alfalfa, 488 g kg-1 DM barley grain, 200 g kg-1 DM soybean meal, 589 g kg-1 DM, corn grain and 20 g kg-1 DM limestone containing predicted metabolizable energy 2.9 Mcal kg-1 DM and containing crude protein 150 g kg-1 DM. The treatments containing different level of ionophore (20 ppm, 25 ppm, 30 ppm and 35 ppm). The experiment period was 21 days. The rumen fluid was collected using stomach tube at 2 and 4 h after morning feeding. The floatation and sedimentation time, methylene blue reduction time, pH, total ammonia nitrogen and total VFA in ruminal samples were determined. The ruminal pH, total VFA, sedimentation and floatation time and methylene reduction time were not significant differences. The effect of lasalocid on ruminal ammonia concentration was significant difference (P<0.05). The results showed that the lasalocid had no effect on ruminal parameters except of ruminal ammonia-N resulted low proteolytic bacteria activity.