In this study, the effect of insulin was investigated in induction of drug resistance to doxorubicin in MCF-7 breast cancer cell line.
MCF-7 cells were pretreated with 10 nM insulin for 48 and 72 hours, respectively. Then, different doses of doxorubicin (1, 5 and 10 μM) were added for an additional 24 hours and cell viability was determined by MTT assay.
Doxorubicin had antitumor effects by reducing the cell survival in a dose-dependent manner. However, the addition of 10 μM doxorubicin in the insulin-treated cells for 72 hours induced significant drug resistance (p<0.01).
The results revealed that insulin could significantly cause the doxorubicin resistance in a time-dependent manner in MCF-7 cells.
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