Assessment of hormonal effects on test tube micropropagation of Saintpaulia 'Pretty Miss Kelly' using micro leaf segments

African violet as one of the important economic ornamental plants could be propagated through tissue culture technique in a short time and limited space. The effect of different growth regulators on shoot production, leave number, root number and rooting time was measured. 'Pretty Miss Kelly' young leaves were collected, and after disinfection, were divided into pieces with dimensions of 1cm*1cm. Therefore, effect of different combination of NAA, BAP, and IAA on shoot regeneration from leaf segments was monitored. Moreover, the effects of various combinations of BAP and AdS has been evaluated on the number of leaves. Subsequently, different root induction mediums including ½ MS with 0.005 mg/l NAA, MS without growth regulators and sterile sand were investigated. The obtained data was analysed by factorial experiment in a completely randomized design with 4 replications. The results showed that 0 mg/l NAA or 0.1 mg/l NAA in combination with 0.1 mg/l BAP and also 0 mg/l IAA in combination with with 0.1 mg/l of BAP are most suitable for shoot production. The best level of AbS and BAP regarding leaf number was inturn 15 mg/l and 0.2 mg/l. A better performance for root induction happened in sterile sand with an average of 25 roots per plantlet. In the case the root induction time, ½ MS with with 0.005 mg/l NAA with an average of 10 days was the best. Therefore, the most appropriate nutrient mediums in various stages for In vitro micrpropagation of African violet from leaf segments was identified.