Investigation the efflux pump gene AdeABC in multidrug resistant clinical isolates of Acinetobacter bumanii

Acinetobacter baumannii is the important cause of nosocomial infections worldwide due to its propensity to rapidly acquire resistance determinants to a wide range of antibiotics and is the major of Multidrug resistant bacteria. There are many different mechanisms for occurrence of MDR in A. baumannii isolates that efflux pumps, beta-lactamases, integrons, insertion sequence(IS) and structural proteins. 79 strains of Acinetobacter baumannii wereisolated from clinical specimens of patients were admitted to Hearth Tehran hospital and diagnosed by biochemical tests. Antibiotic susceptibility of isolates was determined by disc diffusion method according to CLSI 2015. Finally, DNA were extracted and presence of adeA, adeB and adeC genes was evaluated by PCR. The resistance to amikacin, ceftazidime, ciprofloxacin, meropenem, imipenem, tetracycline and trimetoprim were 83.75%,86.25%,82.5%,86.25%,91.25%, 58.75%t 93.75%   respectively. The frequency of adeA,adeB was similar 96.2%  and for adeC gene the frequency was 91.1 % .There was a significant relationship between imipenem and meropenem resistance and frequency of efflux pump genes. According to high prevalence of AdeABC efflux system genes may be play an important role in antibiotic resistance of A. baumannii clinicalisolates specially imipenem and meropenem resistace. Althouth it must be mentioned the role of efflux pumps gene expression in antibiotic resistance.