InvestigationonAntimicrobial Effects of Doped Cu, Al-Zinc Oxide Nanoparticles against Shigella Flexneri

In this study, the synthesis of nanoparticles of doped Copper-zinc oxideandaluminum-zinc oxide by co-precipitationmethod and investigation of structural and morphological properties of related nanoparticles by using Fourier infrared spectrum, X-ray diffraction and transmission electron microscopywere carried out. In FTIR analysis, existence peaks at 1365, 1638 and 2402cm-1attributed to copper in zinc oxide structureand appearance of peaks at 1364, 1740 and 2362 cm-1, showed aluminum in zinc oxide structure. Results obtained from XRD and TEM also confirmed that doping process is successfully done.Resultsshowedthat the dopingof copperand aluminumelements on zinc oxide nanoparticlestructure is successfully performed via y co-precipitation method.In order to investigate the antimicrobial effects of thesenanoparticles, the inhibition zone (mm) and the minimum inhibitory concentration againstShigella flexneri(PTCC 1234) were applied. The results of antimicrobial testsshowed that cu-Zn oxide nanoparticles have more antimicrobial effects on Shigella flexneri(32mm inhibition zone) than Al-zinc oxide (14mm inhibition zone). Additionally, both doped nanoparticles have more antibacterial effect than zinc oxide nanoparticles alone (p≤0/01).Minimum inhabitation concentration of cu and al doped nanoparticles were 70 and 100mg/ml, respectively. Combination of studied nanoparticles with two common biocides (Hypochlorite sodium, hydrogen peroxide) have synergistic effects.