Bioinformatics study of the role of micro RNAs bta-mir-146a, bta-mir-223, bta-mir 21-5p, bta-mir-181 and bta-mir-16a in the cellular signaling pathways of mastitis in dairy cows

 It is reasonable to investigate the signaling pathways in diseases such as mastitis in dairy cattle, which are more likely to be affected by epigenetics.   In this research, 5 microscopic RNA molecules with the accession numbers bta-mir-223, bta-mir 21-5p, bta-mir-181, bta-mir-146a and bta-mir-16a in the sources were identified and extracted from the literatures. Using mirtarbase and targetcan databases, respectively, the confirmed and predicted target genes were extracted for each of the top microRNAs, respectively. Subsequently, the expression of the predicted and confirmed target genes for each of the top microRNAs in the mammary gland tissue was investigated at the NCBI database. In addition, using the DAVID software, cellular signaling pathways were identified in KEGG.   Based on the results, bta-mir-146a microRNA through effects on of 7 proteins, and microRNA bta-mir-223 through effects on 11 proteins and micro RNA bta-mir21-5p through effects on 9 proteins, involved in various cellular signaling pathways, sought to be involved in mastitis in dairy cattle. The results also showed that two bata-mir-181 and bta-mir-16a played an important role in crucial pathways such as the GnRH signaling pathway, Estrogen signaling pathway, Progesterone-mediated oocyte maturation Oxytocin Signaling Pathway and MAPK Signaling Pathway.   This study shows that the microRNAs can be used as molecular markers to dissect molecular etiology of mastitis in dairy cattle.