Evaluation of antioxidant and cytotoxic effects of nanoemulsion of cherry kernel oil on A549 lung cancer and HUVEC normal cells

Free radicals play a role in the pathology of many diseases, such as cancer, diabetes, heart disease, and especially in the aging process. The use of antioxidant agents, especially antioxidants with plant sources, can play a significant role in the treatment of these diseases. The aim of the present study was to prepare the nanoemulsion formulation of sour cherry kernel oil and to investigate its antioxidant and cytotoxic effects on normal and cancerous cells. Nanoemulsion was prepared by the use of polysorbate 80 and polysorbate 20 in water as an emulsion of oil in water. Polyethylene glycol was used as the co-surfactant. Antioxidant effects of concentrations of 1000, 500, 250, 125, 62.5 and 31.25 μg/ml of nanomulsion were investigated on DPPH and ABTS radicals and compared with glutathione as standard. In addition, the cytotoxic effects of nanomulsion on cancer cells A549 and normal HUVEC cells were investigated. The antioxidant results show the potential of nanoemulsion in the removal of radicals of DPPH and ABTS. Furthermore, the results of the cytotoxicity test indicate the greater effects of nanoemulsion on the elimination of cancer cells compared to normal cells. Overall, the results of this study indicate the potentially antioxidant and anti-cancer effects of nanoemulsion of cherry kernel oil, which can be used to prevent and treat many diseases that are associated with the production of reactive oxygen species.