Comparing Applying Different Chromatographic Columns for Acrylamide Analysis in Potato Chips by High-Performance Liquid Chromatography

Acrylamide is a toxic compound produced in some processed foods. The purpose of this research was to provide an easy and reliable method for measuring acrylamide in potato chips by HPLC-PDA. For this purpose, the effect of 5 columns ODS-H-C18 Optimal, Sphere-Image 80-5 ODS 2, Zorbax Hilic Plus, Lichrosorb-100 NH2 and Shodex NH2P-50 4E were investigated on the separation and measurement of acrylamide in chips. The best results were obtained with the use of the new generation of Shodex NH2P-50 4E amino columns, and acrylamide peak was isolated successfully without the need for solid state purification steps. The standard calibration curve was linear in the range of 100-5000 μg / L (R2 = 0.997). Recycling percentages in the range of 95.9% to 102.2%, the detection limit and the limit of value were 9 and 27 μg / Kg respectively. The repeatability of this method was appropriate and the relative standard deviation was 4.39%.