Utilizing encapsulated protease supplement in the diet and its effects on growth, body composition, some blood biochemical factors and activity of intestinal trypsin of beluga (Huso huso)

For enhancing the efficacy of exogenous protease, a chitosan nanoparticles (0.25%) nanoencapsulation-based delivery system was developed. An experiment was conducted for 45 days to evaluate nanoencapsulated protease (NCP) (0.01% and 0.02%) along with 0.25% chitosan nanoparticles against a control diet on productive efficiency, hepato-somatic index, body composition, blood biochemical factors and activity of intestinal protease of the beluga (Huso huso) with mean body weight 10.2±0.3. Different treatments of (CF) were not significantly different from each other (P>0.05), But (WGP), (SGR), (PER) and hepato-somatic index was significantly higher in NCP groups than other groups (P<0.05). The best FCR was in NCP groups and it was significantly lower than other groups (P>0.05). The highest body protein was observed in 0.01 NCP group and it was significantly higher than the control group (P<0.05). The highest body protein was observed in NCP groups (P<0.05). No difference was observed in Blood glucose, albumin and A: G ratio (P>0.05). Blood total protein and globulin were significantly higher in NCP groups compared to the control group (P<0.05). Blood BUN was significantly higher in 0.02 NCP group compared to control group (P<0.05). The highest activity of intestinal trypsin was observed in 0.01 NCP group (P<0.05).