Associated analyses for resistance to sclerotinia stem rot disease in sunflower (Helianthus annuus L.) using retrotransposon-based (REMAP) markers
Sunflower is an important crop that its oil has nutritional and high economic value. Sclerotinia is important fungal disease of sunflower that reduces its growth and yield. In this study, the reaction of 100 oily sunflower lines was studied to 6 fungal isolate of sclerotinia disease. Identification of gene loci related to resistance for disease was done with 120 retrotransposon-based primers (REMAP) locus. The results showed that some sunflower genotypes had the good resistant to sclerotinia disease. 8A×/LC1064C genotype showed low necrosis percentage against two A37 (S. sclerotiorum) and M1 (S. minor) isolates. In population structure analysis, 4 subpopulations were identified (K=4) using STRUCTURE software. As well as in associated analysis based on general and mixed linear models (GLM and MLM) using TASEEL software, 9 and 8 loci were identified respectively that are significant association (P≤0.01) with resistant genes that related to sclerotinia. According to the results, CF-UBC826, 1061LTR-UBC818 and 1061LTR-UBC857 markers were commonly related to the resistant genes to some fungal isolate. Identified markers after validation and tranfering to SCAR markers can be used important in sunflower breeding programs for marker-assisted selection (MAS) and developing resistant cultivars to sclerotinia disease.
Iranian Journal of Field Crop Science, Volume:50 Issue:1, 2019
15 - 29
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