Effect of plant growth regulators on micropropagation of old Abarkooh cypress

In this study proliferation potential of four thousand year old cypress (Cupressus sempervirens L. var. horizontalis (Mill) Gord) using auxins and cytokinins plant growth regulators in in vitro condition was investigated. This study was performed as a factorial experiment and completely randomized design with six replications and each replication were contained three explants. Auxiliary bud explants in two different experiments were cultured in WPM culture medium consisted of cytokinins plant growth regulators (kin, BA, TDZ and 2ip) in five levels (0, 0.1, 0.5, 1 and 2 mg/l) and combination of 0.1 mg/l kin, BA, TDZ and 2ip with 0.01 mg/l IBA. Results showed that the maximum shoot numbers per treatment (2.66) was obtained in WPM culture medium contained 0.01 mg/l IBA + 0.1 mg/l BA and the highest length of shoots (1.85 cm) was obtained in media contained 0.1 mg/l cytokinins and without IBA. For root induction different culture media consisted of WPM, SH and LS contained four concentrations of IBA and NAA (0, 2, 2.5 and 3 mg/l) and also four concentrations (0, 0.5, 1 and 2 mg/l) of Kin was used and only in WPM culture medium contained 2 mg/l kin root was obtained.