Effect of Pseudomonas flourescens FY32 induced bacteria on seed rape (Brassica napus) protein pattern in rapeseed under salinity stress

In this study, inducing ability of endophyte bacteria Pseudomonas flourescens</em> FY32 in growth improving and resistance increasing against salinity stress in strain Hyola308 through proteomic approach was investigated. Experiment was arranged in completely randomized design with three replications in hydroponic culture system. First factor was inoculation of bacteria and non- inoculation (negative control) and the second factor was salinity stress in two levels of NaCl (0 and 300 mM). The results showed that, under salt stress conditions, inoculated plants have better growth characteristics than non- inoculated plants. Protein pattern of leaf tissue was determined by 2 dimensional electrophoresis. After scanning, second dimensional gels was analyzed through Melanie software, then quality and quantity of protein expression in different treatment was compared. 15 spots were indicated changed expression which 4 spots related to oxidative stress, 4 spots related to metabolism pathway and 7 spots related to photosynthesis. Based on the comparison of bacteria and control gels (without bacteria) in two stressed and non-stressed environments, the bacteria increased the resistance of the plant under salinity stress.