Effects of sublethal doses of diazinon on the exspression of immune genes (IL1 and TNFalfa) in zebrafish (Danio rerio)

Diazinon is one of the most important organophosphoric pesticide used in agricultural fields and can effect on immune system. In this study, the effects of sub lethal doses of diazinon (0.8, 1.6 and 3.2 mg/l) on the expression of immune genes (TNFalfa and IL-1) in the liver of zebrafish (Danio rerio) were assessed during 30 days. For this purpose, sampling were done from each treatment at the end of trail. RNA extracted from liver, cDNA synthesized using Suprime ScriptRTase kit and genes expression was assayed using Real-time PCR. The result showed that relative expression of both studied genes significantly increased (P<0.05) in all studied treatments compared to control group. The amount of both studied gene expression was increased in the highest dose and amount of expression was 3.7, 3.9 and 6.6 fold of the control in TNFalfa and 3.96, 4.1 and 5.1 fold of control in IL-1. The results showed that TNFalfa and IL-1 in molecular level as the first cellular response, could be considered as an effective factor for monitoring of ecotoxicological alternations.