Effect of different spraying concentrations of jasmonic acid and titanium dioxide nanoparticles on some physiological traits and antioxidant system activity of Sage (Salvia officinalis L)

In order to evaluate the effects of various concentrations of Jasmonic acid (JA) and nano-titanium dioxide (NTD), as two elicitors, on the activity of antioxidant defensinve system (enzymatic and non-enzymatic) and physiological traits of sage, a factorial experiment in a completely randomized design with three replications was conducted at the University of Zabol. Trial treatments consisted of three concentrations of jasmonic acid (0 (control), 75 and 150 mg.l-1) and titanium dioxide (0 (control), 50 and 100 mg.l-1), respectively. Comparing the mean values showed increasing the concentration of NTD would result in a significant increase of the concentrations of traits examined so that with increasing the concentration of NDT (treatment with 100 mg.l-1), traits such as essential oil, chlorophyll a, chlorophyll b and total chlorophyll, Relative water content, proline, phenol, peroxidase, ascorbate peroxidase, guaiacol peroxidase, superoxide dismutase and catalase would also have an increasing trend compared to control treatment. Also, with increasing the concentration of JA (treatment with 150 mg.l-1), traits such as Relative water content, chlorophyll a, chlorophyll b and chlorophyll total, proline, essential oil, phenol, peroxidase, ascorbate peroxidase, guaiacol peroxidase, superoxide dismutase and catalase would have an increasing trend compared to control treatment. The results of correlation showed a significant positive correlation (P<0.01) between the concetration of Essential oil and phenolic compounds with that of total chlorophyll and also among Antioxidant enzymes. According to the results, using Jasmunic acid and titanium dioxide individually, would have positive effects on the activity of antioxidant system and Physiological traits of Sage.