The Cytotoxic Effects of Testosterone on Gastric Cancer Cell Line (AGS) and Evaluation of Caspase-3, -8, and -9 Activity
Message:
Abstract:
Background and Objectives
Various studies have shown that sex steroids affect cancer cells at cellular and molecular level. In this study, the anti-proliferation effects of testosterone, was investigated on gastric cancer cell line (AGS) and activity level of caspase-3, -8 and -9, was evaluated.
 
Methods
In this laboratory experimental study, AGS cell line, were randomly divided into control group (no exposure to hormone) and groups received testosterone (at concentrations 0.001, 0.01, 0.1, 1, and 10mg/ml). The cytotoxic effect of the extract was measured using MTT assay method. Caspase-3, -8 and -9 activity level was also evaluated using ELISA method. The data were statistically analyzed between groups using one way ANOVA and student’s t-test.
 
Results
Testosterone at the concentrations of 0.1 and 1mg/mL significantly reduced the viability of AGS cells compared to the control group (p<0.001). Exposure of gastric cancer cells to 1mg/mL of testosterone significantly increased the activity level of caspase-3 (p<0.001) and -9 (p<0.05) as compared to the control group, but caused no significant change in the activity level of caspase-8.
Conclusion
The findings of this study showed that testosterone can exert cytotoxic effects on AGS cell line, which this effect is at least partly mediated by caspase-3 and -9.
Article Type:
Research/Original Article
Language:
Persian
Published:
Qom University of Medical Sciences Journal, Volume:13 Issue:6, 2019
Pages:
8 - 17
magiran.com/p2012419  
روش‌های دسترسی به متن این مطلب
اشتراک شخصی
در سایت عضو شوید و هزینه اشتراک یک‌ساله سایت به مبلغ 300,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!