Nanoemulsions of Green Tea Catechins and Other Natural Compoundsfor the Treatment of Urinary Tract Infection: Antibacterial Analysis

Nanoemulsions (NEs) of polyphenon 60 (P60) and cranberry (NE I) and P60 andcurcumin (NE II) were prepared with the aim to enhance anti-bacterial potential and tounderstand the mechanism of anti-bacterial action of the encapsulated compounds. To evaluate the antibacterial potential of the developed NE, microtiter biofilmformation assay was performed. The cytotoxicity analysis was done to assess the toxicity profileof the NEs. Further antibacterial analysis against uropathogenic strains was performed to checkthe developed NEs were effective against these strains. In microtiter dish biofilm formation assay, both NE formulations inhibited the growthmore effectively (Av. % inhibition ~84%) as compared to corresponding aqueous solution(Av. % inhibition ~64%) and placebo (Av. % inhibition ~59%) at their respective minimuminhibitory concentration (MIC) values. Cytotoxicity analysis using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) showed that the formulations were nontoxic toVero cells. The antibacterial studies against uropathogenic resistant strains also showed that NEseffectively inhibited the growth of bacterial strains. From different studies it was concluded that both the NE’s were able to inhibitbacterial strains and could be further used for the treatment of urinary tract infection (UTI). Theantibacterial activity of developed NEs showed that these could be used as alternative therapiesfor the treatment of UTI.