Effects of Combo multi enzyme in the diet on growth factors, survival rate and reproductive performance of gold fish (Carassius auratus gibelio)

The goldfish (Carassius auratus gibelio) is expanded widely as the most famous fish around the world and is considered a good bio-model. Enzymes can eliminate the effects of anti-nutritional agents and improve the performance of fish growth. The diet due to the effect on quality of eggs, it has attracted much attention; new findings express that the addition of multi-enzymes to diet can have effect on gonadal parameters. The aim of this study is to use the Combo multi enzyme in gold fish diet and investigate its effectiveness on growth, survival rate and reproductive performance. To carry out this experiment, the commercial diet (4EF3001, Thailand) was used and the multi-enzyme Combo® (Zubin Zarrin Co) was added to the diet in 5 treatments of 0, 250, 500, 750 and 1000 mg / kg of diet. This multi-enzyme established the best growth factor in the range of 750 and 1000 mg / kg diet, which was significantly different from the control group (P<0.05) however, there was no significant difference in survival percentage between the experimental groups (P>0.05). Also Como multi with the amount of 1000 mg / kg diet established the highest gonadosomatic index, fecundity and fertilization percentage, which was significantly different from the control group (P<0.05) However, in none of the experimental groups, did not establish difference in hatchability, larval survival and sperm motility percentage significantly (P>0.05). In a general conclusion, Combo multi-enzyme with a value of 750 mg / kg diet as the best value of enzyme for growth and 1000 mg / kg diet is considered as the best level for improving reproductive performance.