Aantibacterial Effect of Tragopogon graminifolius Extract on Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli and Salmonella typhi “in vitro”

Sheng (Tragopogon graminifolius) is belongs to the Asteraceae family. In traditional medicine, Sheng is used to treat gastric ulcers, liver dysfunctions, preventing bleeding, pulmonary infections, and wound healing. In this study the antibacterial effect of Sheng extract on Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli and Salmonella typhi was evaluated in laboratory conditions. In this experimental study, antimicrobial activity of Sheng extract on Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli and Salmonella typhi was evaluated by disc diffusion agar and well diffusion agar (diameter of the inhibition zone) methods. Minimum inhibitory concentration (broth microdilution and Triphenyl tetrazolium chloride reagent) and minimum bactericidal concentration (pure plate) of Sheng extract were determined and reported. The results showed that the zone of inhibition diameters increased with increasing concentrations of the extract of Sheng. The highest zone of inhibition (22mm) by well diffusion agar method was related to Staphylococcus epidermidis. The minimum inhibitory concentration of aqueous extract of Sheng for Staphylococcus epidermidis, Bacillus subtilis, Salmonella typhi and Escherichia coli was obtained 64, 256, 256, and 256, respectively. Sheng extract had an appropriate antibacterial effect on the strains studied. It is recommended that further studies be conducted in vitro and in vivo to use this plant to treat infectious diseases and control the growth of pathogenic microorganisms.