Investigation of the Impact of Streptococcus pneumoniae on A549 Pneumocytes
Author(s):
Long He , Da Kang Hu , Shuang Chun Liu , Xin Hua Luo , Lian , Hua Yu , Ying Qu , Jin Hong Yang , Jian Yu , Xiang Yang Li , Wei Wei Shen , Jin Zhang *
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Streptococcus pneumoniae is a leading pathogen worldwide and its pathogenesis remains unclear.
Objectives
The study aimed to investigate the secretion of interleukin-8 (IL-8) and soluble intracellular adhesion molecule-1 (ICAM- 1) from A549 pneumocytes stimulated by different S. pneumoniae strains and the mechanism of blood-derived S. pneumoniae (bd-SP) in invading the blood system.
Methods
Twenty-three clinical strains of S. pneumoniae isolated in 2009 along with ATCC49619 were cultured and suspended in 2018. Then, A549 pneumocytes were resuspended after culture and collection, and inoculated into culture plates. Streptococcus pneumoniae suspensions were then inoculated. Normalsaline was blank control. The plates were incubated for fourand eight hours. Then, the suspensions were collected and centrifuged. The supernatant was analyzed for IL-8 and ICAM-1 by ELISA.
Results
The concentrations of IL-8 were 180.6, 188.5, 223.419.9, and 230.338.6 pg/mL for blank control, ATCC49619, bd-SP, and sd-SP at four-hour stimulation, respectively, and 249.2, 275.7, 224.0 27.8, and 242.3 33.1 pg/mL at eight-hour stimulation. The concentrations of ICAM-1 were 14.8, 12.1, 19.917.2, and 26.128.6 pg/mL for blank control, ATCC49619, bd-SP, and sd-SP at 4 hours, respectively, and 32.6, 150.8, 69.445.1, and 58.730.1 pg/mL at 8 hours.
Conclusions
Streptococcus pneumoniae could upregulate the secretion of IL-8 and ICAM-1 from A549 pneumocytes. No significant difference was found between bd-SP and sd-SP which was in contrast to what was found between clinical S. pneumoniae and ATCC49619. Host response may not be a vital factor for different S. pneumoniae infections.Keywords:
Language:
English
Published:
Jundishapur Journal of Microbiology, Volume:12 Issue: 7, July 2019
Page:
5
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