The Use of Laccase-Catalyzed Oxidation of Poly Phenolic Compounds of Weld in Wool Dyeing

Laccase is a biocompatible compound which is able to catalyze the oxidation of polyphenolic aromatic dyes and graft them to wool. Weld (Reseda Luteolla, L.) as a natural dye, contains polyphenolic aromatics with flavonoid structure and could be a substrate for Laccase. In this research, the effect of laccase on Weld and its use to make a covalant bond between wool and polyphenols compounds of Weld was studied. For this purpose the colourant compounds of Weld were extracted and the effect of laccase on it was studied by UV-Vis spectrophotometry, Size-exclusion chromatography and measuring the changes in the dissolved concentration of the oxygen co-reactant. Kinetic of Laccase-Catalyzed Oxidation of weld was studied and the effect of laccase on weld-dyed wool was surveyed. The results showed that Weld is a suitable substrate for laccase and the use of laccase can improve the dye fixation, and dye fastness against washing.