The effects of induced mild stress of hydrogen peroxide on male gametes

In this research, the effects of induced mild stress of hydrogen peroxide was investigated on bovine male gametes quality. For this purpose, Semen samples were treated with 0, 10, 50, 100 and 200 μmol / L of hydrogen peroxide concentrations, and the parameters related to the total motility, progressive motility, fast motility, survival and quality of sperm cell membrane were recorded and compared in all treatments. Results showed that, increasing of hydrogen peroxide concentration up to 50 μM did not have a significant effect on parameters in compare with control group, but all parameters were decreased when the concentration of Hydrogen Peroxide was going up to 100 and more.