Biosynthesis of citric acid by Yarrowia lipolytica in straw source in laboratory scale

Citric acid is the most important organic acid that used in food industry and pharmaceutical industry. Todays, microbial methods are used to produce citric acid. It is mostly produced by microbial fermentation using Aspergillus niger. In this study we used Yarrowia lipolytica to biosynthesis of citric acid by solid state fermentation from different sources of carbohydrates, such as glucose and straw. At first the culture with different source of carbon were Prepared and yeasts were inoculated and incubated at 250c, 180 rpm for 5 days then concentration of citric acid were measured by spectrophotometer 420 nm. For optimized of conditions such as pH, temperature and, rpm we used Taguchi method. Result that shown that Yarrowia lipolytica could to biosynthesis of citric acid in glucose and straw. In glucose culture the best condition was 300c, pH=5.25, 180 rpm and the amount of citric acid produced was 0.819 g/L. In straw culture the best condition was 320c, pH=4.5, 200 rpm and the amount of citric acid produced was 0/959 g/L. Referring to the results Yarrowia lipolytica had a good potential to biosynthesis of citric acid, in other hands One of the aim of biotechnology, which is the use of agricultural waste and scrap material as a source of food for microorganisms, in this study provided.