Effects of Silver Nanoparticles on Urinary Tract Infections Caused by Gram-negative Bacilli Resistant to Several Antibiotics (MDR)

One of the major problems in hospitals resistance of pathogenic bacteria , including bacteria that cause urinary tract infections(UTI), antimicrobial substances , the problem of increased costs of treatment , treatment failure and mortality rates are increasing.The purpose of this research is to identify multidrug-resistant Gram-negative bacilli of urinary tract infections with antibiotics and the effect of silver nanoparticles on them.Planting 240 clinical samples from patients admitted to two hospitals ( Fatemiyeh , Besat) within 6 months of 1395 a total of 150 gram negative bacilli of urinary tract infections was isolated. To determine antibiotic resistance of these bacteria, disk diffusion method was used, and their susceptibility was evaluated according to the CLSI(clinical and laboratory Standards Institute) . Gram-negative bacilli isolated were prepared and inhibition zone diameter was measured.The most common bacteria Escherichia coli was isolated and lowest frequencies of the Proteus vulgaris. All of the samples to a solution of silver nanoparticles at concentrations 200 ppm 100 ppm and were sensitive. Pseudomonas aeruginosa (30 mm) and Enterobacter aerogenes (27 mm) maximum diameter in the presence of silver nanoparticles showed 800 ppm.

The results show that silver nanoparticles can have an inhibitory effect on all tested Gram-negative bacilli , and the concentration of nanoparticles , the diameter of the growth of Gram-negative bacilli of urinary tract infections resistant to multiple antibiotics increases.Key words: Gram-negative bacilli, Silver nanoparticles, Urinary Tract Infection,Antibiotic Resistance, Hamedan