Different methods using GnRHa on out of season reproductive efficiency in male goldfish (Carassius auratus, Linnaeus 1758)

The present research aims to evaluate effects of different methods of LHRHa implants and injections on some biological properties of semen including sperm density, spermatocrit percentage, pH and duration of sperm motility during spermiogenesis, sperm volume during 21 days after treatment and testosterone hormone changes in the days after implant and injection (Spermiogenesis, 7, 14 and 21) after the treatment in male goldfish broodstock in non-reproduction season. Fish were included in 4 treatments (injection of normal saline 0.7%, EVAc 20 mg / kg body weight LHRHa with 40 micrograms / kg body weight Metoclopramide, cholesterol pellets containing 20 micrograms / kg body weight LHRHa with 40 mg / kg body weight Metoclopramide and 100 mg / kg LHRH with 40 mg / kg body weight Metoclopramide. sperm did not differ significantly between treatments (P ≥0.05). highest spermatocrit percentage was found in treatments EVAc and LHRHa (P ≥0.05). EVAc implants significantly increased sperm volume compared with other treatments during the 21 days after treatment (P ≥0.05). Mobility period in different treatments of male fish broodstock was differed significantly (P ≥0.05), so that mobility period in treatment EVAc mobility was higher than other treatments. PH of different treatments with the control group (Physiology serum injection) showed significant difference so that the lowest rate was observed in the control group (P ≥0.05). Changes in hormone testosterone showed that its rate in Spermiogenesis in EVAc and LHRHa injection treatments showed significant difference compared to other treats in off-season reproduction of goldfish.