The effect of Chrysin inclusion to Beltsville extender on cooling storage of rooster sperm

This study was conducted to investigate the effect of Chrysin inclusion to semen extender on rooster sperm quality during in vitro storage. Semen samples were collected from five 44-week-old male broiler breeders (ROS 308). After primary evaluation (motility >60%), semen samples were pooled and divided into five equal part and each part was diluted with one of the following extenders: 1) control negative extender (without any additive), 2) control positive extender (with 4% DMSO), 3) extender containing 25 μg of Chrysin (Chrysin-25), 4) extender containing 50 μg of Chrysin (Chrysin-50) and 5) extender containing 75 μg of Chrysin (Chrysin-75). Semen quality parameters including total and forward motility, plasma membrane integrity and functionality were assessed in 0, 6, 12 and 24 hours after preservation at 4°C. Based on obtained results, different levels of Chrysin failed to alter total and forward motility, plasma membrane integrity and functionality of spermatozoa during preservation period at 4°C (p≥0.05). Also, the effect of time in all parameters and the effect of time and treatment interaction in sperm plasma membrane functionality were significant. Therefore, Chrysin inclusion failed to ameliorate the negative time-dependent detrimental effects on rooster sperm quality. However, further research is needed to confirm obtained results.