The Effect of Methyl Jasmonat and Resistance to Head Blight Disease on Wheat Caused by Fusarium Graminearum

In this research, interaction between Fusarium graminearum  and wheat including the change in rate of some anti-oxidant enzyme activity and expression level of PR2 (b,1-3gluconase), PR3 (chitinase) andPR4 (wheatwin) genes was studied. Tajan cultivar was used in greenhouse and experimental condition in a completely randomized blocks design with four repetitions. Spraying of aerial parts of plants by methyl- jasmonat at 200, 400 and 600 ppm concentration was done one, three and five days after inoculation with fungal spore of Fusarium graminearum (105 spore per milliliter).Also, inhibition effect of methyl jasmonat on growth of fungal colony was investigated on PDA culture medium.The results showed that the total phenol production and peroxidase, polyphenol oxidase and guaiacol peroxidase reached to highest level three  days after inoculation at concentration of 600 ppm and reduced significantly 5 days after inoculation. Gene expression analysis at three days after inoculation time interval showed the highest and lowest expression level for b, one-three gluconase and chitnase respectively. Assay of growth inhibition effect by methyjasmonat showed that this material has the highest inhibition activity at concentration of 600 ppm. These results indicate that exogenous application of methyl jasmonat as chemical inducer to induce plant resistance against head scab disease could be considered in plant disease management.