Association between Metabolic Syndrome and the Polymorphism of TNF-a-308 G>A Gene in Kerman, Iran

Metabolic syndrome (MetS) is currently prevalent in adults. The main clinical symptoms of MetS include central obesity, hyperglycemia, high triglycerides, low high-density lipoprotein levels, and hypertension. Genetic factors play an important role in the development of MetS. The present study aimed to evaluate the correlation between two gene polymorphisms, including TNF-a -308 G>A (rs 1800629) and IL-6 -174 G>C (rs1800795) with MetS and its components.

This case-control study included 250 participants who were assigned into the case group with MetS (n=125) and control group (n=125). The mean ages of males in the case and control groups were 54.5 and 39.44 years, respectively. Moreover, the mean ages of females in the case and control groups were 53.05 and 33.69 years, respectively. In addition, the number of male and female in the case group was 40 (32%) and 85 (68%), respectively. Additionally, 43 (34.4%) and 82 (65.6%) males and females participated in the control group, respectively. There was no significant difference between the groups regarding gender (P=0.842). In this study, DNA was extracted from the blood, and Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to detect two gene polymorphisms, including TNF-a -308 G>A and IL-6 -174 G>C among participants.

The results obtained from this study showed a significant association between TNF-a-308 G>A and MetS (P<0.05). However, there was no significant association between IL-6 174 G>C and MetS. Additionally, no association was observed between MetS components and the aforementioned polymorphisms.

According to the results, an association was observed between TNF-a-308 G>A gene polymorohism and MetS.