Neuroprotective effects of caffeine against beta-amyliod neurotoxicity: The involvement of glycogen synthase kinase-3β protein

The reduction of glycogen synthase kinase-3β protein level may correlate to the neuroprotective effects of antioxidant agents like caffeine. Therefore, we aimed to evaluate the impact of GSK-3β protein on neuroprotective effects of caffeine in the SHSY5Y cells exposed to beta-amyloid.

We incubated SHSY5Ycells with beta-amyloid 25–35 and caffeine (0.6 and 1mM) for 24h. Cell viability was determined using MTT test. We used the western blotting technique to measure the glycogen synthase kinase-3β and phosphorylated glycogen synthase kinase-3β protein levels.

Caffeine (0.6 and 1mM) diminished beta-amyloid neurotoxicity and attenuated the beta-amyloid effects on the glycogen synthase kinase-3β protein level in a neuronal culture.

Caffeine neuroprotective effects against beta-amyloid may correlate to glycogen synthase kinase-3β protein.</div>