A cross-sectional survey was carried out to determine Seroprevalence of Akabane (AKA) and Bluetongue (BT) viruses in industerial dairy herds in Semnan suburbs, Iran. Serum samples were collected from a total of 184 dairy cattle and tested for antibodies against AKAV and BTV using Competitive-ELISA. The prevalence rates of AKAV and BTV antibodies in cattle were 23.3% (0-75% between herds) and 0%, respectively. The prevalence rates of AKAV antibodies were significantly associated with milking statues (milking or heifer) and abortion history, being higher in milking animals in comparison to heifers (31.3%; P < 0.05) and animals without abortion history than previously aborted cattle’s (34.2%; P < 0.05). The study revealed that AKAV infection is rather high prevalent in dairy herds of Semnan suburbs and this calls for control strategy to be noticed by veterinary services while BTV seems to have less importance in cattle herds of region. Key words: Semnan dairy herds, AKABANE, Bluetongue, Seroprevalence, ELISA.
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