The purpose of this study is to investigate the pro-apoptotic and anti-metastatic potentials of Lippia citriodora</em> alcoholic leaf extract on A2780 ovarian cancer cells and to evaluate the expression of E-cadherin as one of the most important marker in metastasis.
A2780 ovarian cancer cell line was prepared from Pasteur cell bank and cultured in RPMI1640 medium containing 10% FBS and 1% antibiotic. After cell seeding and treatment with different concentrations of extract (10-400 µg/ml), the cells viability and pro-apoptotic potential were examined by MTT assay and acridine orange/ propodium iodide, respectively. Caspase-3 assay and anti-invasive effect were analyzed by migration assay and assessment of E-cadherin expression, respectively. Then, one way ANOVA test was employed for analysis of quantitative data.
Data indicated that L. citriodora</em> alcoholic extract attenuated ovarian cancer cell viability. Acridine orange/ propodium iodide and caspase-3 assays showed that this extract in IC50 concentration (100 µg/ml) induced apoptosis mainly through caspase dependent pathway in the ovarian cancer cells. Migration assay and RT-PCR exhibited that this extract has anti-invasive capability and by up- regulation of E-cadherin prevents the loss of attachment between cancer cells.
The results revealed that L. citriodora</em> alcoholic extract has apoptosis inducing capacity and ability of restoration E-cadherin expression in A2780 cancer cells, which it able to suppress invasive potential of ovarian cancer cells.
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