In the current study, effect of various concentrations of tamoxifen on CTNNBIP1 expression gene in the cancer cells derived from MKN-45 cell line of gastric cancer was considered as a therapeutic potential.
The CSCs derived from the MKN-45 cell line were treated with different concentrations of tamoxifen for 48 hours and then cell survival was evaluated by the Trypan Blue test. After obtaining IC50, we examined the effect of 100 µM tamoxifen on the expression of CTNNBIP1 in CSCs as a therapeutic potential.
Analysis of Real-time RT-PCR data showed that 100 μM tamoxifen in a 48-houred treatment can increase the expression of CTNNBIP1 gene.
According to the results, it was concluded that tamoxifen has an inhibitory effect on the Wnt signal pathway and beta-catenin protein by increasing the expression of the CTNNBIP1 gene.
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