Determination of Dominant Serovars and Molecular Analysis of hly and iap Genes Related to Listeria monocytogenes Strains Isolated from Spontaneous Human Abortions in Tehran
Message:
Abstract:
Background and Aims

Listeria monocytogenes, a gram positive, facultative, intracellular bacterium is the causative agent of listeriosis that is transmitted to human through raw and ready-to-eat foods. The aim of the present study was to determine dominant serovars of L. monocytogenes isolated from spontaneous abortion using phenotypic and genotypic methods.

Materials and Methods

In present study, 258 clinical specimens including placental secretions, vaginal swabs and blood samples from 123 patients with abortion were selected in sterile condition then bacteriological, serological and molecular tests were conducted; dominant serotypes were identified by Multiplex PCR.

Results

Out of 28 (%18.8) isolates of L. monocytogenes 21 (%17.7), 5 (%5.7) and 2 (%3.37) were isolated from placental secretions, vaginal swabs and blood respectively. Maximum and minimum isolated of bacteria related to placental secretions and vaginal swabs with 21 and 2 isolates respectively, of which 14 (%50) 1/2a, 10 (%35.7) 4b and 4 (%14.6) 2c serovars were reported for the first time. All of serovars played a key role in the spontaneous abortion as dominant and common serotypes in Iran. All of the isolates 28 (%22.76) showed hlyA gene and 24 isolates (%19.57) were positive for iap gene and compaired with control group there was significant different between the two groups (P<0.0002).

Conclusion

The present study showed the isolation dominant and common serotypes of L. monocytogenes from spontaneous abortion and demonstrated that the presence of hlyA and iap were effective genes in increasing aggressive and pathogenicity. Serotypes that lacked the iap gene have less pathogenicity and influenced the pathogenesis in mice. It was also concluded that in the absence of access to molecular tests, performing PI-PLC, Congored and in vivo pathogenicity can be effective in detecting pathogenic serotypes from non-pathogenic L. monocytogenes.

Language:
Persian
Published:
Iranian Journal of Medical Microbiology, Volume:13 Issue:2, 2019
Pages:
102 - 113
magiran.com/p2034280  
روش‌های دسترسی به متن این مطلب
اشتراک شخصی
در سایت عضو شوید و هزینه اشتراک یک‌ساله سایت به مبلغ 300,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!