Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture

Three-dimensional (3D) cell culture systems are important because simulating the physiological microenvironment and representing more similarity to in vivo</em> conditions for anticancer drug screening. Taking the advantages of 3D cell culture in cancer therapy field, we have developed the 3D in vitro</em> anaplastic thyroid cancer (ATC) model for determination the cytotoxic dose of "BI-847325" chemotherapy agent in ATC cell lines with different genetic background.
Materials &

C643 and SW1736 ATC cell lines were grown in alginate scaffold. Beads were incubated in medium for one week. Cells were treated with different doses (1–64 μM) of BI-847325 for 24h. The cytotoxic effect of BI-847325 on 3D cultured cell lines was studied by MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay. Survival rate of alginate-encapsulated cells was analyzed by CFSE (5,6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining in effective doses for each of the cell lines.

Cytotoxic effect of BI-847325 anticancer drug was different for two ATC cell lines. Effective doses of BI-847325 for C643 and SW1736 cell lines were at 25μM and 43μM, respectively. CFSE staining analysis confirmed these data.

Altogether, the results of the present study showed that the cytotoxic effect of BI-847325 chemotherapy agent was different for two ATC cell lines. The importance of this subject in regard to the 3D cell culture methods can be useful for researchers in the complementary experiences design in order to achieve the most appropriate chemotherapy drug with the most effective dose.