Frequency of Extend Spectrum β-Lactamases Genes among Klebsiella Pneumoniae Strains Isolated from Patients Admitted to Shahid Beheshti Hospital, Babol City, Iran

Regarding the emergence and increasing of multidrug-resistant strains among Klebsiella pneumoniae nosocomial isolates the therapeutic options for the treatment has been limited. The β-lactamases enzymes are the major defense of gram-negative bacteria against antibiotics. The aim of this study was to the detection of blaCTX-M, blaSHV, and blaTEM genes among K. pneumoniae strains isolated from patients who admitted to Shahid Beheshti hospital of Babol city, Iran, in 2016.

This study was conducted on 50 samples of K. pneumoniae strains isolated from hospitalized patients. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method according to CLSI guidelines. The blaCTX-M, blaSHV, and blaTEM genes were detected by polymerase chain reaction method.

Among studied strains, the prevalence of blaCTX-M, blaSHV, and blaTEM genes were 24 (49%), 44 (88%), and 36 (72%), respectively. In this study, imipenem and nitrofurantoin were more effective than other antibiotics. Also, 100% of strains were susceptible to imipenem.

The prevalence of antibiotic resistance genes detected in this study implies a great concern for the treatment of multidrug-resistant K. pneumonie. Hence, infection control measures, including antibacterial management and identification of resistant isolates for preventing of nosocomial outbreaks have become highlighted.