Evaluation of the culture medium and hormonal treatments on the regeneration of Hymenocrater yazdianus as a medicinal and pasture plant

 Hymenocrater yazdianus is one of the important medicinal and range plant. This plant belongs to Lamiaceae family that grows in arid land regions. In this research mass production of Hymenocrater plant via tissue culture technique was studied. Lateral and apical buds were used as a primary explant for cultivation. For sterilization, Mercuric chloride was used at 0.1% concentration for 2 minutes. After sterilization, explants were washed 3 times with sterilized water containing 0.1% Citric acid for browning prevention of theme. Then all explants transfer to MS medium without any plant growth regulators as an establishment stage. After 4 weeks, explant transported in MS medium containing 0, 0.5, 1, 1.5 mgl-1, Benzyl adenine (BA) and Indole-butyric acid (IBA) concentrations at 0, 0.01 and 0.1 mgl-1 in 12 treatments for proliferation. The results showed, highest shoot number were obtained when we used MS medium at a concentration (1 mgl-1BA and IBA 0.1 mg l-1) In this culture medium we obtained 5.5 shoots in each tube. For rooting stage, MS and LS culture media were studied. In these culture media IBA was used in 0.0, 0.05, 0.1, 1 and 2 mgl-1. The results indicated using MS medium supplemented to 2 mgl-1 95% of explants were rooted.  Finally rooted plantlets were adapted successfully in peat and perlite complex to ratios of 2:1 and in vitro culture plant successfully transferd to the field.