Effect of Iron Oxide and Zinc Oxide Nanoparticles of on Callus Viability of Seedless Barberry

In order to investigate the effect of iron oxide and zinc oxide nanoparticles on callus viability of Berberis vulgaris L., an experiment was conducted in October 2017 at Birjand Agricultural College. Callus were placed on MS/2 medium containing hormonal compounds (TDZ-BAP) and iron oxide and zinc oxide nanoparticles at different concentrations for 2 months. The experiment was conducted in a completely randomized design with 10 different treatments in three replications. The traits were dry weight, weigh weight, callus area, percent of moisture, viability percent, OD485nm. The results of analysis of variance showed that there was a significant difference between treatments for fresh weight, dry weight, moisture percentage content at 5% level, and the comparison of mean treatments showed that (TDZ + 100 PPM zinc oxide nanoparticles) had the highest callus dry weight, fresh weight and moisture percentage (BAP + 75 PPM zinc oxide ) had the highest bioavailability. Orthogonal comparisons between group treatments showed that (TDZ + 100 PPM nano-iron oxide) had the highest and (TDZ + 75 PPM Zn) had the lowest OD. Overall, the results indicated a positive effect of zinc oxide nanoparticles on reducing browning and highest viability of seedless barberry calluses.