Effects of sublethal doses of diazinon on on the expression of vitellogenin (Vtg) gene in females Zebrafish (Danio rerio)

The present study investigates, the effects of different doses of diazinon on the expression of vitellogenin (Vtg) gene in females Zebrafish (Danio rerio</em>). For this purpose, 600 Zebrafish with average weight of 0.15+ 0.01 gr in 4 treatments and 3 replicates for one month were exposed to three doses of 0.8, 0.1 and 3.2 mg /ll of diazinon, and a control group for 30 days. At the end of fexpriment,RNA extracted from liver and brain, cDNA syntesised with Superscript RTase kit and PCR were done using primers relate to Vtg and  Beta-actin as housekeeping gene. The evaluation of the expression of the vitellogenin gene showed significant reduction in diazinon-treated groups compared to the control. In the diazinon-treated groups (0.8, 1.6 and 3.2 mg / L), the expression of the vitellogenin gene was 0.9, 0.96 and 0.39 fold of control, respectively, which showed dose-dependent reduction pattern (P≤0.05). The results of this study indicate that diazinone can have a negative effect on the growth and development of sexual cells in zebra fish.