Evaluation of Prevalence of BKV and JCV DNAs in Renal Allograft Recipients in Guilan Province using Real-time ‌PCR, during 2010-2016

Immunosuppressive drugs that are used for decreasing risk ofacute rejection and renal graft loss can lead to reactivation of latent viruses for example BKVand JCV in either renal allograft or recipients. These viruses can lead to renal graft loss. Thepurpose of this study was to evaluate the quality and quantity of the genome of these twoviruses in the renal recipients’ plasmas for early detection.

In this retrospective descriptive study, at first, DNA extraction test was performedon 102 plasma samples of renalallograft recipients. And then, BKV and JCV DNAs weredetected and quantified byReal-Time PCR.

Fifty four (52.94%)BKV DNA positive and26 (25.50%) JCV DNA positive werefound in 102 recipient plasma samples. Linear range of measurements for BKV and JCVDNAs were within the range of 107-0.596 copies/ μ l and 107-0.528 copies/ μ l respectively.Aftercalculation of genes amounts based on copy/ml in the plasmas, numbers and percent ofpositive cases were highlighted in the four categories. BKV and JCV DNA (Co-infection)were detected in 22 (21.56%) plasma samples.

Real-time PCR is a quantitative and qualitative PCR method that candetectgenome of any type of organisms and their amounts (even in trace amounts),so using of thismethod is very important for early detection of viruses which can cause diseases and graftrejection in renal transplant recipients.