Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells
Message:
Abstract:
Background and Aim

The present study introduces photofunctionalization as a technique for tackling biological aging and increasing the bioactivity of titanium. This in-vitro study evaluated the effects of ultraviolet (UVC) light treatment of titanium surfaces with different time-related changes on the behavior and function of human mesenchymal stem cells (MSCs).

Materials and Methods

MSCs were cultured on nine untreated titanium surfaces (four-week-old surfaces; group 1), nine fresh UVC-treated surfaces (immediately after UV treatment; group 2), and nine three-day-old UVC-treated surfaces (group 3). Cellular proliferation and attachment were measured by MTT. Alkaline phosphatase (ALP) was assessed by total protein extraction, and the enzyme activity was evaluated using a special ALP kit. One-way analysis of variance (ANOVA) and post-hoc Tukey’s test were used to examine the effect of UVC on titanium surfaces.

Results

The mean attachment of MSCs to titanium disks in groups 1 to 3 was 0.118±0.003, 0.103±0.007, and 0.155±0.009, respectively. The mean proliferation of MSCs in groups 1 to 3 was 0.229±0.004, 0.189±0.023, and 0.298±0.020, respectively. The proliferation and attachment in group 3 were significantly higher compared to other groups (P<0.05). Speeds of MSCs growth in groups 1 to 3 were 94%, 81%, and 92%, respectively. The ALP activity of MSCs in groups 1 to 3 was 0.153±0.003, 0.187±0.003, and 0.161±0.003, respectively. The ALP activity in group 2 was significantly higher compared to other groups (P<0.05).

Conclusion

UVC pretreatment of titanium surfaces increases the ALP activity. However, cellular attachment and proliferation were not increased in the present study due to the high probability of laboratory error.

Article Type:
Research/Original Article
Language:
English
Published:
Journal of Research in Dental and Maxillofacial Sciences, Volume:4 Issue:2, 2019
Pages:
7 - 11
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