Study the Survival of Enterobacter cloacae Bacteria in Several Solid Carriers and Effect of Prepared Inoculants on Ggermination and Growth of Wheat

Biofertilizers play major role in sustainable agriculture. Therefore, to provide them, different carriers are used to increase the longevity and survival of the bacteria. The aim of this study was to investigate the survival of Enterobacter cloacae bacteria on different solid carriers during one year. Solid carriers consist of 10 treatments of bagasse, peat, hydrochar, biochar, sawdust and perlite as a single treatment and mixed with perlite (in a ratio of 1:1). In this study, bacterial inoculants prepared with the initial population (109 CFU/g) after storage at room temperature were compared for the survival of the bacteria. The bacterial population was counted at 0, 15, 30, 60, 90, 120, 180, 270 and 365 days. For counting the bacteria in microbial carriers, after dilution series preparation, bacterial suspension was used in strip culture in a plate. In this research, the effects of prepared inoculants on germination and growth of wheat seedlings in sterile conditions in a plate culture at the end of the fourth month were investigated. In these method, characteristics such as shoot and root length, the wet and dry weight of shoot and root, total wet and dry weight of shoot and root were measured.The results of bacterial count showed that among the tested carriers, the most population counted after one year in bagasse carrier (109 CFU/g) and the lowest population was counted in the sawdust, so that after 6 months no alive cells of bacteria were counted in sawdust. Also, the results of germination test and growth wheat seedling growth showed that in most of the measured characteristics the hydrochar and bagasse + perlite in both plate experiment, had reproducible results and they had better means. Concluding, according to the results of this experiment and the convenience and availability of the carriers, bagasse + perlite carrier had the best results in increasing the survival of the bacteria and we suggest this kind of carrier.