The Effect of Intestinal Microbiota Metabolites on HT29 Cell line by Using MTT Method in Patients with Colorectal Cancer

Colorectal cancer is one of the most common malignant tumors, Human guts harbor abundant microbes that adjust many aspects of host physiology.  Increasing studies show that gut microbiota plays a significant role in the incidence and expansion of CRC, as a result of virulence factors, bacterial metabolites, or inflammatory pathways.

In this study, viability of HT29 cells, treated by different time of microbiota metabolits (3,6,12, 18h), was assessed by MTT assay respectively.

Treated cells with microbiota metabolits showed increased viability and proliferation, in time-dependent analysis by MTT assay.

Considering that microbial metabolits is able to induce proliferation and increase cell viability, it seems microbial metabolites imbalance or dysbiosis in the gut due to the dietary or environmental changes or lifestyle risk factors may cause colorectal cancer.