Effects of Rosmarinus Officinalis Plant Extract on Trichomonas Vaginalis Parasites and Candida albicans under Laboratory Conditions: An Experimental Study

As we know metronidazole is a selective treatment for Trichomoniasis, however, it has many side effects, and, on the other hand, it is important to try finding natural alternative treatments.

The aim of this study was to investigate the effects of Rosmarinus officinalis plant extract on Trichomonas vaginalis parasites and Candida albicans under laboratory conditions.

In this experimental study, Rosmarinus officinalis methanolic extracts were prepared. The anti-trichomoniasis effect was evaluated at concentrations of 0.1, 0.01, 0.001, 0.0004, 0.0002, and 0.0001 µg/mL in TYIS33 culture media and resulted data were analyzed by ANOVA.

The results of this study showed that in the concentration of 0.001 at 4 hours, the parasite was spherical and inactivated, while at concentrations of 0.0004, 0.001, 0.01, and 0.1 in 1 hour. The results of this study showed that Rosmarinus officinalis extract in concentration of 100 µg/mL has an inhibitory effect on Candida albicans, so that the highest inhibitory diameter was 18.3 ± 0.9 mm while two other strains exhibit resistance and no inhibitory zones.

The results of this study showed that rosemary extract inhibits the growth of Trichomonas vaginalis and Candida albicans that can be used to treat infections.