INTERACTION EFFECT OF STANOZOLOL AND ENDURANCE TRAINING ON OXIDANT AND ANTIOXIDANT CAPACITY IN LIVER TISSUE OF HEALTHY MALE WISTAR RATS

The purpose of this study was to investigate the interaction effect of endurance training and stanozolol (high dose) on gene expression and activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in liver tissue of healthy male Wistar rats.

Forty male Wistar rats (12-weeks old weighing 289 ± 16 gr) were randomly divided into 4-groups: control (n=10), endurance training (n=10), endurance training+ stanozolol (n=10), and stanozolol (n=10). Endurance training+ stanozolol and stanozolol groups received a weekly intramuscular injection (5mg/kg of body weight) of stanozolol. Endurance training+ stanozolol and endurance training groups were submitted to a progressive running program on a treadmill, for 6 weeks and 5 days per week with 70-75% of VO2max. The content protein of SOD and GPX in liver tissue were measured by ELISA and SOD and GPX mRNA were measured by RT-PCR method. Data were analyzed by one way ANOVA test and statistical significance was defined as P<0.05.

The results showed that SOD mRNA expression in stanozolol group increased significantly Compared to control, endurance training (P<0.001), and endurance training+ stanozolol (P<0.01). In addition, SOD mRNA expression in endurance training+ stanozolol increased significantly compared to the control and endurance training (P<0.01). GPX mRNA expression and activity enzyme of GPX in endurance training+ stanozolol increased significantly compared to stanozolol group (P<0.05). The activity enzyme of SOD in stanozolol, endurance training, and endurance training+ stanozolol increased significantly compared to the control group (P<0.001). The content of liver tissue malondialdehyde in stanozolol group also increased significantly compared to other groups (P<0.001).

It seems that injection of stanozolol increased both of SOD, GPX, and malondialdehyde. Whereas endurance training decreased oxidant effects of stanozolol.