Effect of animal visceral fat on the amount of Hydroxyproline and Histopathological changes on surgical wound healing in male rat
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background

High costs of treatment and rehabilitation lead to increased tendency of researchers using the natural and  cheaper compounds. Sheep visceral fat contains various types of fatty acids with. anti-inflammatory and therapeutic effects for wound healing. The aime of  this study is to investigate the effect of visceral fat treatment on wound healing in male rats .

Methods

In this experimental study 50 adult male Sprague-Dawley rats with the relative weight of 250-300 g were selected.All rats were randomly divided into 2  groups with 25 rats in each grupe, treated with 10ml melted visceral fat, and control group asses  then full thickness excisional wounds  on the back skin under surgical anesthesia., respectively. The  of evaluation of the wound size was investigated by imaging  using an accurate caliper and Image J software. On days 5, 10, 12, 15 and 21, tissue biopsy from both control and treatment groups was performed to compare wound healing factors. The biopsy sample at21th day  was check the amount of hydroxyproline precipitate in tissue. Finally, the results were compared by one way ANOVA and Tukey tests.

FINDINGS

Based on morphometric findings, on the 10th day of treatment ,the  mean ulcer size of the treatment group with visceral fat was 43.6±0/44 mm2 while the mean size of  the ulcer in control group was 98/5±0/07mm2, which has shown to have the least amount of  wound contraction. Also on the 5th day, the wound contraction of the treatment group , was twice as high as the control group(p<0.05). The histological examination shows the mean 2.74±0.17 points of restoration parameters score in treatment group  compared to the control group with a score of 1.42±0.16 points . Also, the difference between the Hydroxyproline concentration of the healthy skin and the scarring wounds in treatment group (3.69±0.23 mg/g) was less than  the control group (6.08±0.32 mg/g) (p<0.05).

Conclusion

According to the evidence of this study, the effect of animal visceral lipid on healing of full tickness skin lesions is because of anti inflammatory role.

Language:
Persian
Published:
Journal of Babol University of Medical Sciences, Volume:21 Issue: 1, 2019
Pages:
349 to 356
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